Produced:Article Title: Corticotropin-releasing hormone signaling from prefrontal cortex to lateral septum suppresses interaction with familiar mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-c-fos antibody produced in rabbit Abcam #ab190289; RRID:AB_2737414 Anti-GFP antibody produced in chicken AVES Labs #GFP-1020; RRID:AB_10000240 Anti-GFP polyclonal antibody, Alexa Fluor 488 Thermo-Fisher Scientific #A21311; RRID:AB_221477 Anti-RFP antibody produced in rabbit Rockland Antibody #600-401-379; RRID:AB_2209751 Anti-mCherry antibody produced in goat Biorbyt #orb153320 Anti-GABA antibody produced in guinea-pig Abcam #ab17413; RRID:AB_443865 Donkey anti-goat IgG (H+L) secondary antibody, Alexa Fluor 568 conjugate Thermo-Fisher Scientific #A11057; RRID:AB_2534104 Goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 568 conjugate Thermo-Fisher Scientific #A11011; RRID:AB_143157 Goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A32733; RRID: AB_2633282 Goat anti-guinea-pig IgG (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A21450; RRID:AB_2735091 Goat anti-mouse IgG1 (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A21240; RRID:AB_2536165 Goat anti-rat IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate Thermo-Fisher Scientific #A48262; RRID:AB_2896330 Goat Anti-chicken IgG (H+L) secondary antibody, Alexa Fluor 488 Conjugate Thermo-Fisher Scientific #A11039; RRID:AB_142924 Chemicals, peptides, and recombinant proteins Stressin-1 Tocris #1608 Antalarmin Tocris #2778 DMSO Sigma-Aldrich #D8418 CNO Cayman Chemical #16882 CGP 55845 Tocris Cat# 1248 SR 95531 Tocris Cat# 1262 ISH probe Mm-Crh ACD Bio #316091 ISH probe mCherry-C2 ACD Bio #431201-C2 Experimental models: Organisms/strains C57BL/6J Mus musculus Jackson Laboratories RRID:IMSR_JAX:000664 B6(Cg)-Crhtm1(cre)Zjh/J Jackson Laboratories RRID:IMSR_JAX:012704 B6-Crhr1tm4(cre)Jde Jan Deussing RRID:MGI:6281608 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Jackson Laboratories RRID:IMSR_JAX:007909 Recombinant DNA AAV2/DJ hSyn.FLEX.mGFP.2A.Synatophysin-mRuby Stanford vector core #1930 / Addgene #71760 AAV2/9 EF1a.DIO.hChR2(E123T/T159C).eYFP Addgene #35505-AAV9 HSV hEF1a.LSIL.GFP (HT) Massachusetts General Hospital #RN406 HSV hEF1a-Cre (HT) Massachusetts General Hospital #RN242 (Continued on next page) e1 Cell 186, 4152–4171.e1–e8, September 14, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER AAV2/8 hSyn.DIO.hM4D(Gi)-mCherry Addgene #44362-AAV8 AAV2/8 hSyn.DIO.mCherry Addgene #50459-AAV8 AAV2/5-hSyn-DIO-hM3D(Gq)-mCherry Addgene #44361-AAV5 AAV2/5 hsyn.DIO.mCherry Addgene #50459-AAV5 AAV2/1 syn.FLEX.GCaMP6f.WPRE.SV40 Addgene #100833-AAV1 PX552 Addgene #60958 rAAV-U6-shRNA1(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2784 rAAV-U6-shRNA2(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2785 rAAV-U6-shRNA3(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2786 rAAV-U6-shRNA(scramble)-CMV-EGFP-SV40-polyA Brain VTA #PT-0916 rAAV-CMV-CRH-P2A-EGFP-WPRE-hGH-polyA Brain VTA #PT-2827 AAV2/9 CMV-DIO-(mCherry-U6)-shRNA(anti-Crh) Brain VTA #PT-2787 AAV2/9 CMV-DIO-(mCherry-U6)-shRNA(scrambled) Brain VTA #PT-2788 AAV2/9 hSyn.FLEX.dsRed-shRNA(vGAT) Addgene Addgene #67845 AAV 2/9 hSyn-flex-dsRed-shRNA(scrambled) Addgene Addgene #71383 AAV2/5 DIO.mGFP University of North Carolina #AV4310i AAV2/2 CAG.FLEX.ArchT-tdTomato University of North Carolina Addgene #28305 AAV2/2 CAG.FLEX.tdTomato University of North Carolina Addgene #28306 AAV2/1 syn.GCaMP6f.WPRE.SV40 Pennsylvania University #AV-1-PV2822 AAV2/9 syn.CRF1.0 Yulong Li Lab Ref. 51 AA2/2 hSyn1.hChR2(H134R)-mCherry.WPRE University of Zurich #V-124 AA2/2 hSyn1.mCherry.WPRE Addgene #114472-AAV2 Software AxoGraph AxoGraph 1.6.4 PRISM 9 Graphpad 9.0.1 (128) Microsoft Office Word Microsoft 2019 16.56 Microsoft Office Exel Microsoft 2019 Adobe Illustrator Adobe 2020 v24.1 FIDJI GPL v2 2.3.0/1.53f MATLAB Mathworks 2018 Python 3.10.2 Guppy Lerner Lab79 1.1.4 Leica Application Suite X Leica v3.7.4 ANY-maze Stoelting Co. 4.99 Doric Neuroscience Studio Doric 5.4.1.23 ll
Recombinant:Article Title: Corticotropin-releasing hormone signaling from prefrontal cortex to lateral septum suppresses interaction with familiar mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-c-fos antibody produced in rabbit Abcam #ab190289; RRID:AB_2737414 Anti-GFP antibody produced in chicken AVES Labs #GFP-1020; RRID:AB_10000240 Anti-GFP polyclonal antibody, Alexa Fluor 488 Thermo-Fisher Scientific #A21311; RRID:AB_221477 Anti-RFP antibody produced in rabbit Rockland Antibody #600-401-379; RRID:AB_2209751 Anti-mCherry antibody produced in goat Biorbyt #orb153320 Anti-GABA antibody produced in guinea-pig Abcam #ab17413; RRID:AB_443865 Donkey anti-goat IgG (H+L) secondary antibody, Alexa Fluor 568 conjugate Thermo-Fisher Scientific #A11057; RRID:AB_2534104 Goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 568 conjugate Thermo-Fisher Scientific #A11011; RRID:AB_143157 Goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A32733; RRID: AB_2633282 Goat anti-guinea-pig IgG (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A21450; RRID:AB_2735091 Goat anti-mouse IgG1 (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A21240; RRID:AB_2536165 Goat anti-rat IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate Thermo-Fisher Scientific #A48262; RRID:AB_2896330 Goat Anti-chicken IgG (H+L) secondary antibody, Alexa Fluor 488 Conjugate Thermo-Fisher Scientific #A11039; RRID:AB_142924 Chemicals, peptides, and recombinant proteins Stressin-1 Tocris #1608 Antalarmin Tocris #2778 DMSO Sigma-Aldrich #D8418 CNO Cayman Chemical #16882 CGP 55845 Tocris Cat# 1248 SR 95531 Tocris Cat# 1262 ISH probe Mm-Crh ACD Bio #316091 ISH probe mCherry-C2 ACD Bio #431201-C2 Experimental models: Organisms/strains C57BL/6J Mus musculus Jackson Laboratories RRID:IMSR_JAX:000664 B6(Cg)-Crhtm1(cre)Zjh/J Jackson Laboratories RRID:IMSR_JAX:012704 B6-Crhr1tm4(cre)Jde Jan Deussing RRID:MGI:6281608 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Jackson Laboratories RRID:IMSR_JAX:007909 Recombinant DNA AAV2/DJ hSyn.FLEX.mGFP.2A.Synatophysin-mRuby Stanford vector core #1930 / Addgene #71760 AAV2/9 EF1a.DIO.hChR2(E123T/T159C).eYFP Addgene #35505-AAV9 HSV hEF1a.LSIL.GFP (HT) Massachusetts General Hospital #RN406 HSV hEF1a-Cre (HT) Massachusetts General Hospital #RN242 (Continued on next page) e1 Cell 186, 4152–4171.e1–e8, September 14, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER AAV2/8 hSyn.DIO.hM4D(Gi)-mCherry Addgene #44362-AAV8 AAV2/8 hSyn.DIO.mCherry Addgene #50459-AAV8 AAV2/5-hSyn-DIO-hM3D(Gq)-mCherry Addgene #44361-AAV5 AAV2/5 hsyn.DIO.mCherry Addgene #50459-AAV5 AAV2/1 syn.FLEX.GCaMP6f.WPRE.SV40 Addgene #100833-AAV1 PX552 Addgene #60958 rAAV-U6-shRNA1(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2784 rAAV-U6-shRNA2(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2785 rAAV-U6-shRNA3(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2786 rAAV-U6-shRNA(scramble)-CMV-EGFP-SV40-polyA Brain VTA #PT-0916 rAAV-CMV-CRH-P2A-EGFP-WPRE-hGH-polyA Brain VTA #PT-2827 AAV2/9 CMV-DIO-(mCherry-U6)-shRNA(anti-Crh) Brain VTA #PT-2787 AAV2/9 CMV-DIO-(mCherry-U6)-shRNA(scrambled) Brain VTA #PT-2788 AAV2/9 hSyn.FLEX.dsRed-shRNA(vGAT) Addgene Addgene #67845 AAV 2/9 hSyn-flex-dsRed-shRNA(scrambled) Addgene Addgene #71383 AAV2/5 DIO.mGFP University of North Carolina #AV4310i AAV2/2 CAG.FLEX.ArchT-tdTomato University of North Carolina Addgene #28305 AAV2/2 CAG.FLEX.tdTomato University of North Carolina Addgene #28306 AAV2/1 syn.GCaMP6f.WPRE.SV40 Pennsylvania University #AV-1-PV2822 AAV2/9 syn.CRF1.0 Yulong Li Lab Ref. 51 AA2/2 hSyn1.hChR2(H134R)-mCherry.WPRE University of Zurich #V-124 AA2/2 hSyn1.mCherry.WPRE Addgene #114472-AAV2 Software AxoGraph AxoGraph 1.6.4 PRISM 9 Graphpad 9.0.1 (128) Microsoft Office Word Microsoft 2019 16.56 Microsoft Office Exel Microsoft 2019 Adobe Illustrator Adobe 2020 v24.1 FIDJI GPL v2 2.3.0/1.53f MATLAB Mathworks 2018 Python 3.10.2 Guppy Lerner Lab79 1.1.4 Leica Application Suite X Leica v3.7.4 ANY-maze Stoelting Co. 4.99 Doric Neuroscience Studio Doric 5.4.1.23 ll
In Situ Hybridization:Article Title: Corticotropin-releasing hormone signaling from prefrontal cortex to lateral septum suppresses interaction with familiar mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-c-fos antibody produced in rabbit Abcam #ab190289; RRID:AB_2737414 Anti-GFP antibody produced in chicken AVES Labs #GFP-1020; RRID:AB_10000240 Anti-GFP polyclonal antibody, Alexa Fluor 488 Thermo-Fisher Scientific #A21311; RRID:AB_221477 Anti-RFP antibody produced in rabbit Rockland Antibody #600-401-379; RRID:AB_2209751 Anti-mCherry antibody produced in goat Biorbyt #orb153320 Anti-GABA antibody produced in guinea-pig Abcam #ab17413; RRID:AB_443865 Donkey anti-goat IgG (H+L) secondary antibody, Alexa Fluor 568 conjugate Thermo-Fisher Scientific #A11057; RRID:AB_2534104 Goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 568 conjugate Thermo-Fisher Scientific #A11011; RRID:AB_143157 Goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A32733; RRID: AB_2633282 Goat anti-guinea-pig IgG (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A21450; RRID:AB_2735091 Goat anti-mouse IgG1 (H+L) secondary antibody, Alexa Fluor 647 conjugate Thermo-Fisher Scientific #A21240; RRID:AB_2536165 Goat anti-rat IgG (H+L) secondary antibody, Alexa Fluor 488 conjugate Thermo-Fisher Scientific #A48262; RRID:AB_2896330 Goat Anti-chicken IgG (H+L) secondary antibody, Alexa Fluor 488 Conjugate Thermo-Fisher Scientific #A11039; RRID:AB_142924 Chemicals, peptides, and recombinant proteins Stressin-1 Tocris #1608 Antalarmin Tocris #2778 DMSO Sigma-Aldrich #D8418 CNO Cayman Chemical #16882 CGP 55845 Tocris Cat# 1248 SR 95531 Tocris Cat# 1262 ISH probe Mm-Crh ACD Bio #316091 ISH probe mCherry-C2 ACD Bio #431201-C2 Experimental models: Organisms/strains C57BL/6J Mus musculus Jackson Laboratories RRID:IMSR_JAX:000664 B6(Cg)-Crhtm1(cre)Zjh/J Jackson Laboratories RRID:IMSR_JAX:012704 B6-Crhr1tm4(cre)Jde Jan Deussing RRID:MGI:6281608 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Jackson Laboratories RRID:IMSR_JAX:007909 Recombinant DNA AAV2/DJ hSyn.FLEX.mGFP.2A.Synatophysin-mRuby Stanford vector core #1930 / Addgene #71760 AAV2/9 EF1a.DIO.hChR2(E123T/T159C).eYFP Addgene #35505-AAV9 HSV hEF1a.LSIL.GFP (HT) Massachusetts General Hospital #RN406 HSV hEF1a-Cre (HT) Massachusetts General Hospital #RN242 (Continued on next page) e1 Cell 186, 4152–4171.e1–e8, September 14, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER AAV2/8 hSyn.DIO.hM4D(Gi)-mCherry Addgene #44362-AAV8 AAV2/8 hSyn.DIO.mCherry Addgene #50459-AAV8 AAV2/5-hSyn-DIO-hM3D(Gq)-mCherry Addgene #44361-AAV5 AAV2/5 hsyn.DIO.mCherry Addgene #50459-AAV5 AAV2/1 syn.FLEX.GCaMP6f.WPRE.SV40 Addgene #100833-AAV1 PX552 Addgene #60958 rAAV-U6-shRNA1(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2784 rAAV-U6-shRNA2(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2785 rAAV-U6-shRNA3(CRH)-CMV-EGFP-SV40-polyA Brain VTA #PT-2786 rAAV-U6-shRNA(scramble)-CMV-EGFP-SV40-polyA Brain VTA #PT-0916 rAAV-CMV-CRH-P2A-EGFP-WPRE-hGH-polyA Brain VTA #PT-2827 AAV2/9 CMV-DIO-(mCherry-U6)-shRNA(anti-Crh) Brain VTA #PT-2787 AAV2/9 CMV-DIO-(mCherry-U6)-shRNA(scrambled) Brain VTA #PT-2788 AAV2/9 hSyn.FLEX.dsRed-shRNA(vGAT) Addgene Addgene #67845 AAV 2/9 hSyn-flex-dsRed-shRNA(scrambled) Addgene Addgene #71383 AAV2/5 DIO.mGFP University of North Carolina #AV4310i AAV2/2 CAG.FLEX.ArchT-tdTomato University of North Carolina Addgene #28305 AAV2/2 CAG.FLEX.tdTomato University of North Carolina Addgene #28306 AAV2/1 syn.GCaMP6f.WPRE.SV40 Pennsylvania University #AV-1-PV2822 AAV2/9 syn.CRF1.0 Yulong Li Lab Ref. 51 AA2/2 hSyn1.hChR2(H134R)-mCherry.WPRE University of Zurich #V-124 AA2/2 hSyn1.mCherry.WPRE Addgene #114472-AAV2 Software AxoGraph AxoGraph 1.6.4 PRISM 9 Graphpad 9.0.1 (128) Microsoft Office Word Microsoft 2019 16.56 Microsoft Office Exel Microsoft 2019 Adobe Illustrator Adobe 2020 v24.1 FIDJI GPL v2 2.3.0/1.53f MATLAB Mathworks 2018 Python 3.10.2 Guppy Lerner Lab79 1.1.4 Leica Application Suite X Leica v3.7.4 ANY-maze Stoelting Co. 4.99 Doric Neuroscience Studio Doric 5.4.1.23 ll
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